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LC3B antibody

• 貨號：	gtx127375
  應用范圍：	Flow cytometry/FACS, ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
  適應物種：	Human, Mouse, Pig, Rat
  宿主：	Rabbit
  克隆性：	否

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LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.

Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.

Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:100.
Blue: Hoechst 33342 staining.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.

HepG2 cells were untreated or treated with 3 μM thapsigargin for 16 and 24 hrs. Whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:1000.

LC3B antibody detects LC3B protein in HCV-infected samples by Western blot analysis.

  A. 20 µg Huh7 whole cell lysate/extract (un-infected)
  B. 20 µg Huh7 whole cell lysate/extract (HCV-infected)
  LC3B antibody (GTX127375) dilution: 1:1500
 

LC3B antibody detects MAP1LC3B protein by Western blot analysis.

A. 20 µg Huh7 whole cell lysate/extract (untreated)
B. 20 µg Huh7 whole cell lysate/extract (3uM-Thapsigargin treatment for 12hr)
LC3B antibody (GTX127375) dilution: 1:1500

LC3B antibody detects LC3B protein by western blot analysis. Un-treated (-) and treated (+, 50μM Chloroquine treatment for 48 hr) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) at a dilution of 1:500.The beta Actin was used as internal control (GTX10564, 1:50000) shown at the bottom panel.

LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at a dilution of 1:1000.

LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.

Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min.
Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunoprecipitation of LC3B protein from U87-MG whole cell extracts using 5 μg of LC3B antibody (GTX127375).

Western blot analysis was performed using LC3B antibody (GTX127375).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

LC3B antibody detects LC3B protein at cytoplasm in mouse brain by immunohistochemical analysis.

Sample: Paraffin-embedded mouse brain.
LC3B antibody (GTX127375) diluted at 1:500.

LC3B antibody (GTX127375) detects LC3B protein by flow cytometry analysis.

Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: LC3B antibody (GTX127375) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

LC3B antibody detects MAP1LC3B protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
15 % SDS-PAGE
LC3B antibody (GTX127375) dilution: 1:1000

LC3B antibody detects MAP1LC3B protein by Western blot analysis.

A. 50 µg Rat brain lysate/extract
15 % SDS-PAGE
LC3B antibody (GTX127375) dilution: 1:1000

LC3B antibody detects LC3B protein in Thapsigargin-treated samples by Western blot analysis.

Upper panel: LC3B antibody (GTX127375)
Lower panel: Beta-actin antibody (GTX110564)A. 30 µg HepG2 whole cell lysate/extract (untreated)
B. 30 µg HepG2 whole cell lysate/extract (3 µM Thapsigargin treatment for 12 hr)
15 % SDS-PAGE
LC3B antibody (GTX127375) dilution: 1:1000
Beta-actin antibody (GTX110564) dilution: 1:20000

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